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Moodle
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Related Assignment:
Learner Objectives
You should be able to:
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Describe the action of restriction enzymes in recombinant
DNA
technology and fingerprinting techniques.
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Define the term Restriction Fragment Length Polymorphisms
(RFLPs)
and describe how they are separated by electrophoresis and identified
using DNA probes.
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Explain the effects of mutation and inheritance of DNA
that provide
the theoretical basis for the use of DNA fingerprinting, also called
DNA
profiling, as a tool for the identification of individuals or
determination of the relationships of organisms.
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Interpret simulated DNA evidence and decide on the guilt
or
innocence of the suspect.
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Briefly describe how a segment of DNA is sequenced using
the Sanger, or cycle sequencing, method.
Web Resources for this Discussion
DNA
Interactive
Collection
of Biotechnology Animations from Cold Spring Harbor
The animations dealing with PCR
, Southern
Blotting, and Cycle
Sequencing are especialy good.
Sequence
for
Yourself from The
Nova Program: Cracking the Code of Life. This animation is rather
detailed, but you have been exposed to all the basic concepts you need
to understand how genes are sequenced.
This is another possibility for extra credit. Watch the
entire
program and write it up as a
Media Watcher" extra credit project.
Some Applications of DNA Fingerprinting:
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DNA
Detective from The Dolan DNA Learning Center, Cold Spring Harbor
Laboratory.
Animation of DNA fingerprinting and summaries of several
real
examples solved using DNA fingerprint techniques
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Electrophoresis
animation from DNA
Interactive.
Select "Techniques at the bottom of the screen
Select "Sorting and Sequencing" from the top of the next screen.
Select the "Gel Electrophoresis" animation.
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New
Methods of DNA Profiling using PCR and STRs (Short Tandem Repeats).
DNA Interactive:
Applications
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Select "Human Identification from the bottom of the
first
screen
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Select "profiling" from the top of the second screen.
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Roll your mouse over the right hand circle near the top
of the
screen ("Today's DNA Profile") and click to select the animation.
The Sanger Method of DNA Sequencing, AKA "Cycle Sequencing"
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