Lab Resources for
Polymerase Chain Reaction (PCR), DNA Fingerprinting, and DNA Sequencing

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Web Crossing

Web Resources for this Lab:

• DNA Replication (Making sister chromatids during the S phase of the cell cycle)
• Chose "Copying the Code" toward the bottom of the screen
• then select "puting it together" from the top of the next screen.
• Then choose the "Transcription animation"
• Transcription DNA --> RNA
• Chose "Copying the Code" toward the bottom of the screen
• then select "puting it together" from the top of the next screen.
• Then choose the "Transcription animation"
• Translation mRNA --> Protein
• Chose "Reading the Code" toward the bottom of the screen
• then select "puting it together" from the top of the next screen.
• Then choose the "Translation animation"

This is another possibility for extra credit. Watch the entire program and write it up as a Media Watcher" extra credit project.

Polymerase Chain Reaction

Select Manipulation from the left-hand menu

Choose "Techniques" at the bottom of the next screen

Choose "Amplifying" at the top of the next screen

Two animations, "Making many copies of DNA" and "PCR animation" are very useful.

Some Applications of DNA Fingerprinting:

• DNA Detective from The Dolan DNA Learning Center, Cold Spring Harbor Laboratory.

  Animation of DNA fingerprinting and summaries of several real examples solved using DNA fingerprint techniques

• Electrophoresis animation from DNA Interactive.

Select "Techniques at the bottom of the screen
Select "Sorting and Sequencing" from the top of the next screen.
Select the "Gel Electrophoresis" animation.

• Web Crossing Supplement - Electrophoresis and DNA Analysis
• Southern Blotting: Gel Transfer from Access Excellence (Similar to the Web Crossing Supplement above)
• DNA Fingerprinting Animation from Cold Spring Harbor Laboratory
• New Methods of DNA Profiling using PCR and STRs (Short Tandem Repeats). DNA Interactive: Applications
• Select "Human Identification from the bottom of the first screen
• Select "profiling" from the top of the second screen.
• Roll your mouse over the right hand circle near the top of the screen ("Today's DNA Profile") and click to select the animation.

The Sanger Method of DNA Sequencing

• DNA Sequencing from Kimball's Biology Pages
• DNA Sequencing from the Cold Spring Harbor Biology Animation Library
• Sequence for Yourself from NOVA
• Lecture Activity: DNA Sequencing ----- ANSWER