Blanke Laboratory Homepage

Professor Blanke Information Page

• Blanke, S. R. (2006) Portals and pathways: Principles of bacterial toxin entry into cells, Microbe 1, 26-32.

• Nossa, C. W., & Blanke, S. R. (2006) Modification of a mammalian cell protein in the presence of [³²P-adenylate] NAD: Evidence for Helicobacter pylori ADP-ribosylation activity, in press in Infection and Immunity.

• Ye, D., & Blanke, S. R. (2006) Bacterial toxins as Cellular Modulators, in Press in “Molecular Paradigms of Infectious Disease: A Bacterial Perspective.”

• Ye, D., & Blanke, S. R. (2006) Structure-function relationships underlying a novel class of Helicobacter pylori VacA mutants with antitoxin activity, submitted manuscript under revision.

• Cover, T.L., & Blanke, S. R. (2005) Helicobacter pylori VacA as a paradigm for toxin multifunctionality, Nature Reviews Microbiology 3, 320-332.

• Blanke, S. R. (2005) Micro-managing the executioner: Pathogen targeting of mitochondria, Trends in Microbiology 13, 64-71.

• Willhite , D.C. , & Blanke, S.R. (2004) Helicobacter pylori Vacuolating cytotoxin enters cells, localizes to the mitochondria, and induces mitochondrial membrane permeability changes correlated to toxin channel activity, Cellular Microbiology 6, 143-154.

• Willhite , D.C. , Cover, T.L., & Blanke, S.R. (2003) Cellular vacuolation and mitochondrial cytochrome c release are independent outcomes of Helicobacter pylori vacuolating cytotoxin activity that are each dependent on membrane channel formation, J. Biol. Chem. 278, 48204-48209.

• Patel, H. K., Patel, R. M., Willhite, D. C., Williams, C. L., Marty, K. B., Buford-Fieth, J., Torres, E., & Blanke, S. R. (2002) Plasma membrane cholesterol modulates cellular vacuolation induced by the Helicobacter pylori vacuolating cytotoxin, Infection and Immunity 70, 4112-4123.

• Willhite, D. C., Ye, D., & Blanke, S. R. (2002) FRET Microscopy of the Helicobacter pylori vacuolating cytotoxin within mammalian cells, Infection and Immunity 70, 3824-3832.

• Ye, D. & Blanke, S. R. (2002) Domain complementation reveals the importance of intermolecular monomer interactions for Helicobacter pylori VacA cellular activity, Molecular Microbiology 43, 1243-1253.

• Marty, K., Williams, C. L., Guynn, L., Benedik, M., & Blanke, S. R. (2002) Characterization of a cytotoxic factor in the culture filtrates of Serratia marcescens, Infection and Immunity 70, 1121-1128.

• Blanke, S. R., & Ye, D. (2001) Alternative mechanisms for protein release, in Helicobacter pylori: Physiology and Genetics, H.L.T. Mobley, G.L. Mendz, S.L. Hazell (eds.), 227-238; ASM Press; Washington D. C.

• Patel, H. K., & Blanke, S. R. (2001) Cellular Hijacking: Exploitation of membrane trafficking patterns by bacterial toxins, Bull. Biochemistry and Biotechnology 14, 20-48.

• Ye, D. & Blanke, S. R. (2000) Mutational analysis of the Helicobacter pylori vacuolating toxin amino-terminus: Identification of amino acids essential for cellular vacuolation, Infection and Immunity 68, 4354-4357.

• Patel, R. M., Lindsey, L., & Blanke, S. R. (2000) In vitro evolution of RNA-based aptamers to probe toxin active sites, International Journal of Medical Microbiology 290, 62-63.

• Willhite, D. C., Ye, D., Gutierrez, A., Williams, C. L., Patel, H., Marty, K. B., Mahoney, D. T., & Blanke, S. R. (2000) Intracellular life and times of the Helicobacter pylori vacuolating toxin, Bull. Biochemistry and Biotechnology 13, 35-53.

• Ye, Dan, Willhite, D. C., & Blanke, S. R. (1999) Identification of the minimal intracellular vacuolating domain of the Helicobacter pylori vacuolating cytotoxin. J. Biol. Chem. 274, 9277-9282.

• Vinion, A. D., McClain, M. S., Czajkowsky, D. M., Iwamoto, H., Ye, D., Cao, P., Schraw, W., Szabo, G., Blanke, S. R., Shao, Z., & Cover, T. L. (1999) A dominant negative mutant of Helicobacter pylori vacuolating toxin (VacA) inhibits VacA-induced cell vacuolation, J. Biol. Chem 274, 37736-37742.

• Lodaya, R., Blanke, S. R., Collier, R. J., & Slama, J. T. (1999) Photoaffinity labeling of diphtheria toxin fragment A with 8-Azidoadenosyl nicotinamide dinucleotide, Biochemistry 38, 13877-13886.

• Willhite, D. C., & Blanke, S. R. (1999) Turning sword into plowshare: Engineering bacterial toxins as molecular delivery systems. Bull. Biochemistry and Biotechnology 12, 15-22.

• Willhite, D. C., & Blanke, S. R. (1998) Soluble expression and one-step purification of recombinant Bacillus anthracis protective antigen, Protein and Peptide Letters 5, 273-278.

• Fu, H., Blanke, S. R., Matheakis, L. C., & Collier, R.J. (1997) Selection of diphtheria toxin active-site mutants in yeast. Rediscovery of glutamic acid-148 as a key residue. Adv. Exp. Med. Biol. 419, 45-52.

• Berti, P. J., Blanke, S. R., & Schramm, V. L. (1997) Transition state structure for the hydrolysis of NAD catalyzed by diphtheria toxin, J. Am. Chem. Soc. 119, 12079-12088.

• Blanke, S. R. (1997) Bacterial toxins engineered as drug delivery systems targeting mammalian cells, Seminars in Frontier Technology 22, 23-25.

• Kaul, P., Shen, W. -H., Silverman, J., Blanke, S. R., Huynh, P. D., Finkelstein, A., & Collier, R. J. (1996) Roles of Glu 349 and Asp 352 in membrane insertion and translocation by diphtheria toxin, Protein Science 5, 687-692.

• Blanke, S. R., Milne, J. C., Benson, E. L., & Collier, R. J. (1996) Fused polycationic peptide mediates delivery of diphtheria toxin A chain to the cytosol in the presence of anthrax protective antigen, Proc. Natl. Acad. Sci. USA 93, 8437-8442.

This page last updated April 8, 2006