Single mutations in cytochrome b
Click here for Table 1b, Multiple mutations
Table 1a. Single mutations in the cyt b subunit of the bc1 complex from prokaryotes and eukaryotes and their salient effects
# Residue Growth Phenotype Properties References
Prokaryotes/Eukaryotes Prokar./Eukar. Prokaryotes /Eukaryotes
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1 I33/I17F * /Gly+ * /DiR, HQR [5]
2 W45/W30C * /Gly+ * /FuR, HQR [19]
3 I46/N31K * /Gly+ * /AnR, DiR, HQR [5]
4 G48D/G33D Ps-/Gly- Affects assembly /Affects assembly [7,20,21]
G48V/G33 Ps-/* Modified bL and bH, Qi- /* [7]
rG48A/rG33A Ps+/Gly+ No effect /FuR, HQR, Shifted bH [7,20,21]
5 A52V/G37V Ps+/Gly+ AnR, Affects Qi kinetics /AnR, FuR, HQR [7,5]
A52D/G37V(M. m.) Ps-/na Affects assembly /AnR, FuR [4,25]
A52/A37V,G(S. p.) * /na * /AnR, DiR [4]
6 Q58L/Q43 Ps+/* Modified bL /* [7]
7 H97D,N/H82 Ps-/* bL and bH absent /* [7]
8 S102A/S87 Ps+/* No effect /* [7]
9 F104I/F89 Ps+/* No effect /* [7]
10 L106P/M91(fs) Ps+/Gly- MyR, MucR /nd [7,22]
11 H111Y,N,D/H96 Ps-/* bH absent /* [7]
12 R114Q/K99 Ps-/* Affects assembly /* [15]
R114A,R/K99 Ps-/* Inactive /* [23,15]
R114K/K99 Ps+/* Lower Em of bH /* [15]
13 G119/G104(fs) * /Gly- * /Affects assembly [22,24]
Y118/rY103I * /Gly+ * /nd [22,24]
14 W129A/W114 Ps+/* Qi kinetics perturbed /* [15]
W129F/W114 Ps+/* Low level of bc1 /* [15]
15 M140G,C/I125 Ps+/* No effect /* [8]
M140R/I125 Ps-/* Occupied Qo, QH2 ox- /* [8,26]
M140I,V,W/I125 Ps+/* MyR, StR /* [8]
M140S/I125 Ps+/* MyR /* [8]
M140L,N,D/I125 Ps+/* StHS /* [8]
16 G141/A126T * /Gly+ * /MyR, FuR [6]
17 F144R,K/F129 Ps-/* Ion pair at Qo, QH2 ox- /* [26,8]
F144S,T/F129 Ps+/* MyR, Partially occupied Qo,
Slow QH2 ox /* [26,27,8]
F144V/F129 Ps+/* MyR, StR, Partially occupied Qo, /* [26,27,8]
F144I,G,L/F129L Ps+/Gly+ MyR, StR, Empty Qo, Slow QH2 ox /MyR, StR, MuR, Modified bL [26,27,6]
F144N/F129 Ps+/* MyR, Empty Qo, Slow QH2 ox, /* [8,27,5]
F144A,H/F129L(C. r.) Ps+/na MyR /MyR, MuR [4,8]
F144Y,W/F129 Ps+/* StR /* [8,26,27]
F144C/F129 Ps+/* MyR, StR /* [8]
18 G146A,V/G131S Ps-/Gly- Modified bL, Empty Qo, QH2 ox- /Affects assembly [28,5]
19 Y147F/Y132C(C. r.) Ps+/na Slow QH2 ox /MyR, MuR [29,4]
Y147V/Y132 Ps+/* MyR, Slow QH2 ox /* [29]
Y147A,S/Y132 Ps-/* Occupied Qo, QH2 ox- /* [29]
20 V148/C133Y * /Gly- * /MyR, Modified bL,
Qo and Qi red affected [5,30]
V148/rC133S,F,D,H * /Gly+ * /nd [5]
21 G152T,D,E,H,R/G137R Ps+/Gly+ MyR /MyR, MuR [8,5]
G152K,C,Y,I,F,L,V,N
/G137S(C. r.) Ps+/na StR, MyR /MyR, MuR [4,8]
G152P/G137E Ps-/Gly- Affects stability /StR, MyR, MuR, Uncoupled and
affects stability (FeS) [7,8,13,16,31]
G152S/G137V Ps+/Gly- MyR, MucR /MyR [7,8,5]
G152A/rG137A Ps+/Gly+ StR /Slow growth [7,8,5]
22 Q153/Q138 Stop * /Gly- * /nd [5]
Q153/rQ138Y,E,L * /Gly+ * /nd [5]
23 M154V/M139 Ps+/* MyR /* [29]
24 S155C/S140 Ps-/* nd /* [32]
25 W157A,S/W142R Ps+/Gly- Low (30%) bc1 activity /MyHS, Qo and Qi red affected [32,33]
W157/rW142K,T * /Gly+ * /MyR, Low bc1 activity [33]
W157/rW142S * /Gly+ * /Minor effect [33]
26 G158A,S/G143A(M. m.) Ps+/na MyR /MyR, MuR [4,8,27]
G158T,C,N,D,E,H,R,L,I,V,W
/G143 Ps-/* Empty Qo, QH2 ox- /* [27]
27 T160F/T145 Ps-/* Empty Qo, Slow QH2 ox /* [8,34]
T160R,S,C,L,Y/T145 Ps+/* MyHS, Partially occupied Qo,
Low bc1 activity /* [3,34]
T160A,V Ps+/* Minor effects /* [23]
28 I162G,A,L,F/I147F Ps+/Gly+ No effect /StR [32,5]
29 T163A,C/T148M(M. m.) Ps+/na StR /StR [8,4]
T163V,L,I/T148 Ps+/* MyHS /* [8]
T163R/T148 Ps-/* Empty Qo site, 5 % bc1 activity /* [8,26]
T163F,P/T148 Ps-/* Affects assembly /* [8]
T163S/T148 Ps+/* No effect /* [8,14]
30 L165G,A/L150F Ps+/Gly- No effect /nd [5,32]
31 F166Y,W,L,G,V/F151C Ps+/Gly- No effect /nd [5,32]
32 G167S/S152 Ps+/* No effect /* [32]
33 P170S/P155 Ps+/* No effect /* [32]
34 G171S/F156 Ps+/* No effect /* [32]
35 S175C/N159 Ps+/* No effect /* [32]
36 G182S/G167 Ps+/* Slow QH2 ox /* [32]
37 A185C/S170 Ps+/* No effect /* [32]
38 H198N,Y/H183 Ps-/* bH and bL absent /* [7]
39 P202L/P187 Ps+/* No effect /* [7]
40 V209A/V194 Ps+/* FuS /* [23]
V209T/V194 Ps+/* No effect /* [23]
41 H212D/H197 Ps-/* bH absent /* [7]
H212Q/H197 Ps-/* Affects assembly /* [23]
42 I213V/L198F Ps+/Gly+ Qi kinetics perturbed /FuR [23,5]
43 H217A/H202 Ps-/* QH2 ox- /* [35]
H217R/H202 Ps+/* More stable SQi- at Qi, Qi
kinetics perturbed /* [36]
H217L/H202 Ps-/* Unstable SQi- at Qi, Qi- /* [36]
H217D/H202 Ps+/* Unstable SQi- at Qi, Qi
kinetics perturbed /* [36]
H217Q/H202 Ps-/* Affects assembly /* [23]
44 S218A/I203 Ps+/* No effect /* [23]
45 T219A/H204(fs) Ps+/Gly- Slow QH2 ox, Modified Em of bL /Affects assembly [23,37,38]
T219/rH204Y * /Gly+ * /HQR [37,38,39]
46 G220V/G205 Ps+/* Qi kinetics perturbed /* [23]
47 N221I/S206L Ps+/Gly- Slow bH ox /HQR, Shifted bH, Qi perturbed [5,21,23]
N221/rS206T,V * /Gly+ * /HQR [37,38,39]
N221S/S206 Ps+/* No effect /* [23]
48 N222A/S207 Ps+/* Qi kinetics perturbed /* [23]
49 N223Q/N208 Ps+/* Em of bH shifted /* [23]
N223V/N208 Ps+/* Qi kinetics perturbed /* [23]
N223A,D/N208 Ps+/* No effect /* [23]
50 P224A/P209 Ps+/* No effect /* [23]
51 F244L/M221K Ps-/Gly- Affects assembly /An and Fu binding
modified, Qi- [5,21,23,40]
F244/rM221E,Q * /Gly+ * /DiR, HQR [20,21]
52 F248L/F225L,S Ps-/Gly+ Affects assembly /DiR, HQR [5,23]
53 K251M/K228M(K. l.) Ps+/na AnR, Slow bH to Qi kinetics /AnR [4,35]
K251I/K228I Ps+/Gly+ AnR, Slow bH to Qi kinetics /AnR [5,35]
54 D252A/D229 Ps-/* AnR,bH ox blocked /* [35,25]
D252N/D229 Ps+/* AnR, Qi- /* [35,25]
D243H,E(R. r.)/D229 Ps+/* AnR, Qi site defective /* [8,41]
55 A255/G232D(M. m.) * /na * /AnR, HQR, FuR [4]
56 F263/L240(fs) * /Gly- * /nd [22, 24]
57 N279Y/N256Y Ps+/Gly+ StR, MyR /StR, MyR, MuR, Affects
stability (FeS) [5,23]
N279/N256K * /Gly+ * /MuR, StR [13]
N279/N256I * /Gly+ * /MuR, StR, Affects stability (FeS) [13]
58 I292F/I269 Ps-/* QH2 ox- /* [23]
59 P294S/P271 Ps+/* Slow QH2 ox /* [23]
P294L/P271 Ps-/* Affects assembly /* [23]
60 E295D,G/E272 Ps+/* StR, Slow QH2 ox /* [23]
E295Q/E272 Ps+/* StR, QH2 ox- /* [23,25]
61 W296L/W273 Ps+/* StR, MyR, Slow QH2 ox /* [23]
Y297F/Y274 Ps+/* Slow QH2 ox /* [23]
63 F298/L275F * /Gly+ * /MyR [5]
F298/L275S,T * /Gly+ * /MuR,MyR [5]
64 L305D/L282F Ps+/Gly- Slow QH2 ox /Affects assembly [5,23]
L305A/rL282I,L Ps-/Gly+ Affects assembly /nd [23,42]
L305/rL282C,T * /Gly+ * /Slow growth [23,42]
65 K329G/K288 Ps+/* Slow QH2 ox /* [23]
K329A/K288 Ps-/* Affects assembly /* [23]
66 G332S/G291 Ps+/* Slow QH2 ox /* [23]
G332I/G291 Ps-/* Qo site blocked /* [23]
G332D/G291 Ps+/* Slow QH2 ox /* [23]
67 V333A,M/V292 Ps+/* StR /* [8,23]
V333G,R,L/V292 Ps+/* No effect /* [8]
68 M336/L295F(M. m.) * /na * /MyR, StR [4]
69 Y358F,T/F318 Ps+/* No effect /* [32]
70 F367L/K326 Ps+/* No effect /* [32]
71 W368R/F327 Ps+/* No effect /* [32]
72 A371Q/V330 Ps+/* No effect /* [32]
73 D373V/N332 Ps+/* Lower bc1 activity /* [32]
74 F374T/F333 Ps+/* No effect /* [32]
75 V376/L335Stop * /Gly- * /Affects assembly [43]
76 G381/G340E * /Gly- * /Affects assembly [5]
G381/rDG340 * /Gly+ * /nd [42]
G381/rG340A,V * /Gly+ * /nd [42]
77 Y389/Y348(fs) * /Gly- * /Affects assembly [43]
Y389/rY348I * /Gly+ * /nd [43]
D390/rV349C * /Gly+ * /nd [43]