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Table 1b. Multiple cyt b mutations obtained as revertants of non functional cyt b mutants

#	Residue
	Initial mutation			Growth Phenotype			Properties			References
	Prokaryotes/Eukaryotes		Prokar./Eukar.		Prokaryotes		/Eukaryotes
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3	N31K
	I46+I59/N31K+I44Ta(K. l.)	*   /na			*			/AnR				[4]
5	G37V
	A52+A76/G37V+A61V		*   /Gly+		*			/AnR				[5]
10	M91(fs)											
	L106+A107+V108+Y109/	
	M91Y+V92G+M93N+F94V		*   /Gly+		*			/nd				[22]
	L106+A107/M91Y+V92G		*   /Gly+		*			/nd				[22]
13	G104(fs)	 									
	L116+Y117+Y118/
	L101Y+Y102I+Y103I		*   /Gly+		*			/nd				[22,24]
18	G131S												
	G146+A283/G131S+G260A		*   /Gly+		*			/Slow growth			[5]
19	Y147A or S 
	Y147A+M154V/Y132+M139		Ps+ /*		MyR, Restores partial
									QH2 ox		/*				[29]
	Y147S+M154S/Y132+M139		Ps+ /*		MyR, Restores partial 
									QH2 ox		/*				[29]
20	C133Y
	V148+M145/C133Y+L130M		*   /Gly+		*			/Slow growth			[5]
	V148+L191/C133Y+I176S		*   /Gly+		*			/Slow growth			[5] 
	V148+P384/C133Y+H343T		*   /Gly		*			/Slow growth			[42] 
	V148+G141/C133Y+A126T		*   /Gly+		*			/Ts, Slow growth		[5]
	T126+Y133
	G141+V148/T126+Y133F,S		*   /Gly+		*			/nd				[5]
	G141+V148/T126+Y133N,D		*   /Gly+		*			/MyR				[5]
	G141+V148/T126+Y133C	 	*   /Gly+		*			/nd				[5]
	G141+V148+P150/
	T126+Y133+V135I			*   /Gly+		*			/Slow growth			[5]
	G141+M145+V148/
	T126+L130M+Y133			*   /Gly+		*			/Slow growth			[5]
	G141+V148/T126+C133H		*   /Gly+		*			/Slow growth			[5]
21	G137E
	G152+V148/G137E+C133S		*   /Gly+		*			/MyR, Low bc1 activity		[5,16,31]
	G152+M140/G137E+I125T		*   /Gly+		*			/Slow growth			[5]
	G152+F156/G137E+H141Y		*   /Gly+		*			/Slow growth			[5]
	G152+I162/G137E+I147F		*   /Gly+		*			/Low bc1 activity		[5,16]
	G152+F166/G137E+F151L		*   /Gly		*			/Slow growth		[	5]
	G152+N279/G137E+N256K		*   /Gly+		*			/StR, MyR, MuR, Slow growth, 																	Affects stability (FeS) 	[5,13,16]
	G137V
	G152+F156/ G137V+H141Y		*   /Gly+		*			/Slow growth			[5]
25+26	W157G+G158Wa/W142+G143		Ps- /*			nd			/*				[32]
26+28	G158I+I162Ga/G143+I147		Ps- /*			nd			/*				[32]
29	T163F
	T163F+G182S/T148+G167		Ps+ /*		Low bc1 activity
							Affects stability (FeS)		/*				[14]
40+42	V209I+I213Va/V194+L198		Ps+ /*		No effect			/*				[23]
45	H204(fs) 	
	T219+G220/H204S+G205C		*   /Gly+		*			/HQR				[37,38,39]
47	S206L
	N221+N223/S206L+N208K,Y		*   /Gly+		*			/FuR, HQR,Ts, 
											bH perturbed by Fu		[37,38,40]
	N221+W45/S206L+W30C		*   /Gly+		*			/HQR, Ts			[37,38,39]
52	F248+V249/F225L+I226Fa		*   /Gly+		*			/DiR, HQR			[4]
53+54	K251D+D252Ka/K228+D229		Ps+ /*		AnR, Qi kinetic perturbed	/*				[23]
.	L253+F254+A255/DL230+DI231
	+T232Sa(K. l.)			*   /na			*			/AnR, HQR			[4]
56	L240(fs)
	V259+L260+L261+G262/
	F236I+M237Y+L238V+I239N		*   /Gly+		*			/nd				[22,24]
62+63	Y297+F298/Y274N+L275Fa		*   /Gly+		*			/MuR, MyR			[5] 
64	L282F
	L305+N279/L282F+N256I		*   /Gly+		*			/Slow growth			[42]
	L305+I304/L282F+I281F		*   /Gly+		*			/Slow growth			[42]
	L305+A328/L282F+D287H		*   /Gly+		*			/Slow growth			[42]
76	G340E
	G381+M383/G340E+G342G		*   /Gly+		*			/Slow growth			[42]
	G381+E386/G340E+E345G		*   /Gly+		*			/Slow growth			[42]
	G381+K329/G340E+K288N		*   /Gly+		*			/Slow growth			[42]
77	Y348(fs)
	D390+W391+I392/
	V349C+L350F+M351N		*   /Gly+		*			/nd				[43]
	D390+W391+I392+L394/
	V349C+L350F+M351N+Q353T		*   /Gly+		*			/nd				[43]
	Y387+P388+Y389/
	V346Y+P347T+Y348I		*   /Gly+		*			/nd				[43]
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Footnotes:
 K. l.: K. lactis;  M. m. : M. Musculus;  S. p.: S. pombe;  C. r.: C. reinhardtii;  R.r.: R. rubrum;  
*Not available; nd: not determined; na: not applicable; r: revertant; 
Position 37 is V, 218 is T in R. capsulatus; but A and S in R. sphaeroides respectively;
QH2: Ubihydroquinone; SQ: Ubisemiquinone; Qo; Ubihydroquinone oxidation site; Qi: Ubiquinone reduction site; 
bH: cyt bH heme; bL: cyt bL heme; Em: Redox midpoint potential; ox: oxidation; red: reduction; 
Di: Diuron; HQ: 2-hepthyl-4-hydroxyquinoline N-oxide (HQNO); An: antimycin; Fu: Funiculosin; My: Myxothiazol; Mu: Mucidin; 
R: Resistant; S: Sensitive; HS: Hypersensitive;
Ts: temperature sensitive; Gly+/-: Respiratory competent/deficient; Ps+/-: Photosynthetically competent/incompetent;
fs: frame shift; FeS: ‘Rieske’ FeS protein; 
aMultiple mutations; D : deletion.