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Course Web Site: http://www.life.uiuc.edu/mcb/301
Course Instructor:
Dr. Gail Grabner
232 Burrill Hall
333-7704
ggrabner@life.uiuc.edu |
Laboratory Coordinator:
Renee L. Alt
241 Burrill Hall
333-4306 (Office), 333-8057 (Lab)
rlalt@uiuc.edu |
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Office Hours (Dr. Grabner)
M, W, F: 10:00 AM - 11:00 AM
(or by appointment) |
Office Hours (Ms. Alt)
M-Th: 10:30 AM - 11:30 AM
(or by appointment) |
Course Description: Laboratory emphasizing the fundamentals of microbiology. Topics include growth, isolation, and identification of bacteria; antibiotic resistance; Southern blot; ELISA; conjugation and transposon mutagenesis.
Credit:3 credit hours. Students must register for one lab-discussion and one lecture section.
Prerequisite: Students are expected to have taken MCB 250 and MCB 251 or course equivalents prior to enrolling in MCB 301. Students should also have credit or concurrent registration in MCB 252, MCB 253, and MCB 300.
Required Course Materials:
- “Experimental Microbiology: Laboratory Exercises for MCB 301, Spring 2008 ”. Gail K. Grabner, Tina M. Knox and Jack Ikeda.
- “Biology Laboratory Notebook” Carbonless with duplicate, numbered pages
- Ruler and Calculator, required at every lab and exam
Course Policies:
All students are assumed to have read and understood the “Code of Policies and Regulations Applying to All Students,” University of Illinois, and will be expected to act accordingly.
The Code is available online at: http://www.admin.uiuc.edu/policy/code/index.html
Attendance Policies:
Consult with Lab Coordinator, Renee Alt, about any course absences or conflicts.
All students are assumed to have read and understood attendance policies printed in the lab manual.
Disabilities and Religious Observances:
Please contact the course coordinator during the first week of classes to make requests for disability accommodations or observation of religious holidays.
Spring 2008 Syllabus: Word PDF
Course Syllabus
Laboratory Schedule
An experiment highlighted in bold type indicates a pre-lab is due for that exercise prior to the lab period
| Date |
Experiment |
Lab Manual Pages |
Day 1
Mon Jan 14
Tu Jan 15 |
Course introduction
1: Streak Plate
2: Spread Plate
3: Luminescent bacteria |
i-xvii
1-9
10-14
15-19
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Day 2
Wed Jan 16
Th Jan 17 |
Course introduction
1, 2, 3: Evaluations
4: Simple Stain
5: Gram stain |
i-xvii
8, 13, 18
20-32
27-33 |
Mon Jan 21
Tu Jan 22 |
HOLIDAY |
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Day 3
Wed Jan 23
Th Jan 24 |
6: Wet mount, Darkfield
7a-b: Temperature effects, 1
|
36-46
47-55 |
Day 4
Mon Jan 28
Tu Jan 29 |
7a-b: Temperature effects, 2
8a-d: Enzymes and adaptation, 1 |
52, 53
56-67 |
Day 5
Wed Jan 30
Th Jan 31 |
8a-d: Enzymes and adaptation, 2
9: Bioinformatics
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61-65
Handout |
Day 6
Mon Feb 4
Tu Feb 5 |
10: Snyder test, 1
11a-c: Biochemical Tests, 1
12: Growth curve, lecture |
68-70
71-112
113-125 |
Day 7
Wed Feb 6
Th Feb 7 |
10: Snyder test, 2
11a-c: Biochemical Tests, 2
13a: Lactic acid bacteria enrichment
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69
71-112
126-137 |
Day 8
Mon Feb 11
Tu Feb 12 |
13b: Lactic acid bacteria isolation
14a: Sporeformers enrichment
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134
138-160 |
Day 9
Wed Feb 13
Th Feb 14 |
13c: Lactic acid bacteria observation
14b: Sporeformers isolation, 1
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135
150-151 |
Day 10
Mon Feb 18
Tu Feb 19 |
14b: Sporeformers isolation, 2
15a: Identification isolation |
152-153
162-177 |
Day 11
Wed Feb 20
Th Feb 21 |
14c: Sporeformers post-enrichment, 1
15b: Identification purification, 1
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154-155
171-172 |
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Th Feb 21 |
OPEN HOURS BEGIN, SEE SCHEDULE
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Day 12
Mon Feb 25
Tu Feb 26 |
14C: Sporeformers post-enrichment, 2
15b: Identification purification, 2 |
155-158
172 |
Day 13
Wed Feb 27
Th Feb 28 |
15b: Identification purification, 1
15c: Identification dichotomous keys; inoculate tests, 1
16: pGLO transformation, 1
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172
173-174
178-181 |
Day 14
Mon Mar 3
Tu Mar 4 |
15c: Identification inoculate/score tests, 2
16: pGLO transformation, 2 |
173-174
180 |
Day 15
Wed Mar 5
Th Mar 6 |
15c: Identification dichotomous keys; inoculate tests, 3
17a: ELISA fluid exchange
17b: ELISA analysis, 1
18a: Southern blot electrophoresis
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173-174
183-192
189-191
202-217 |
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Fri Mar 7 |
OPEN HOURS END
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Day 16
Mon Mar 10
Tu Mar 11 |
17b: ELISA analysis, 2
17c: Agglutination (demo)
18b: Southern blot transfer |
191
193-201
218-224 |
Day 17
Wed Mar 12
Th Mar 13 |
18a: Southern blot hybridization
19a: Purple non-sulfer bacteria enrichment
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225-230
243-252 |
Mon Mar 17 to
Sun Mar 23 |
SPRING BREAK
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Day 18
Mon Mar 24
Tu Mar 25 |
18b: Southern blot washing
19b: Purple non-sulfer bacteria isolation, 1
20: Analysis of sore throat, 1 |
231-232
248
253-259 |
Day 19
Wed Mar 26
Th Mar 27 |
18e: Southern blot detection
20: Analysis of sore throat, 2
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233-236
254-256 |
Day 20
Mon Mar 31
Tu Apr 1 |
18f: Southern blot analysis
19b: Purple non-sulfer bacteria isolation, 2
20: Analysis of sore throat, 3
21a-b: MIC determination, 1 |
237
249
257
260-267 |
Day 21
Wed Apr 2
Th Apr 3 |
19c: Purple non-sulfer bacteria observation
21a-b: MIC determination, 2
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249
264-265 |
Day 22
Mon Apr 7
Tu Apr 8 |
19d: Purple non-sulfer bacteria characterization, 1
22a: Transposon mutagenesis conjugation |
250-251
268-281 |
Day 23
Wed Apr 9
Th Apr 10 |
19d: Purple non-sulfer bacteria characterization
22b: Transposon mutagenesis selection, 1
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251
273 |
Day 24
Mon Apr 14
Tu Apr 15 |
22b: Transposon mutagenesis selection, 2 |
274
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Day 25
Wed Apr 16
Th Apr 17 |
22c: Transposon mutagenesis characterization, 1
Lab Practical Review
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275-277
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Day 26
Mon Apr 21
Tu Apr 22 |
22c: Transposon mutagenesis characterization, 2
Lab Practical Review
Lab clean up & drawer inventory |
278-279
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Day 27
Wed Apr 23
Th Apr 24 |
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, Brightfield microscopy, streak plate
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Day 28
Mon Apr 28
Tu Apr 29 |
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key |
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Lecture Schedule
| Date |
Topic(s) |
| January 18 |
Pure culture, aseptic technique, plating techniques, cell wall structure, staining, microscopy |
| January 25 |
Effects of temperature; Enzymes and Adaptation |
| February 1 |
Biochemical Tests; Snyder |
| February 8 |
Exam 1
Aseptic Technique, Pure culture, Plating, Luminescent bacteria, Staining, Microscopy, Effects of temperature, Enzymes and Adaptation |
| February 15 |
Lactic acid bacteria; Spore-forming bacteria |
| February 22 |
Sporeformer metabolism and post enrichment; Identification and dichotomous keys |
| February 29 |
Bacterial transformation and pGLO; ELISA and agglutination; Southern digest and electrophoresis |
| March 7 |
Exam 2
Biochemical tests, Snyder test, Growth curve, Lactic acid bacteria, Sporeformers, Identification |
| March 14 |
Southern transfer, probe synthesis, and hybridization; Photosynthesis and purple non-sulfer bacteria |
| March 21 |
Spring Break |
| March 28 |
Southern washing, detection and analysis; Observation and characterization of purple non-sulfer bacteria; Analysis of sore throat |
| April 4 |
MIC determination; Conjugation and pRL27 plasmid; Transposon mutagenesis and cell types |
| April 11 |
Transposon mutagenesis selection, counter selection and characterization |
| April 18 |
Lab practical review; ICES forms for Dr. Grabner |
| April 25 |
Exam review |
| Finals Week |
Exam 3
pGLO, ELISA and agglutination, Southern blot (a-f), Purple non-sulfur bacteria, Sore throat, MIC, Transposon mutagenesis |
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