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Latest Update: Sunday, January 14, 2007
Course number: MCB 493
CRN: 47430
3 Credit Hours
Topic: Cellular Microbiology & Disease. This course emphasizes the cell biology of infectious diseases, using cellular, molecular, and animal models. We will stress the molecular cross-talk that drives host-pathogen interactions, state-of-the-art approaches for investigating host and microbial cell and molecular biology, latest paradigms in host cell biology, and, the evolutionary basis by which pathogens can manipulate cytoskeleton, membranes, organelles, cell cycle, and signaling in eukaryotic cells. Please contact Steve Blanke with questions or concerns at sblanke@life.uiuc.edu.
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Meeting Time: Tuesday, Thursday: 2:00 – 3:20 pm
Room: Armory 133
Text: Cellular Microbiology, 2nd Edition (2005; Cossart, Boquet, Normark, and Rappuoli)
Publisher: ASM Press
ISBN: 1-55581-302-X
***Assigned readings and/or handouts
Instructor: Dr. Steven R. Blanke
Office hours:
Tuesday: 8:00 – 8:30 pm
Wednesday: 4:30 – 5:00 pm
Thursday: 3:20 pm – 3:40 pm
Almost any other time that is scheduled (including Saturdays and Sunday afternoon) - must schedule at least 48 h ahead of time.
Office: 302A Burrill Hall
Telephone: 217-244-2412
Email: sblanke@life.uiuc.edu
Goals of Course:
** TO EXPLORE THE EUKARYOTIC-PROKARYOTIC INTERFACE **
1. To explore the molecular cross-talk that drives host-pathogen interactions.
2. To introduce state-of-the art approaches for investigating the cell biology of infectious disease.
3.
To introduce the latest paradigms in host cell biology, as related to infection
4.
To explore the evolutionary basis by which pathogens can manipulate eukaryotic cells
5. To understand the basis of how manipulation of host cell biology contributes to disease.
6. For students to investigate the latest research on cellular microbiology.
Class schedule:
| Module 1:
| Fundamentals of Host-Pathogen Interactions (Chapters 1-2).
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Class |
1 |
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Intro. to Cellular Microbiology; Fundamental principles of infectious diseases – I; Pathogens of the Week #1 |
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Class |
2 |
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Fundamental principles of infectious diseases - II |
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Class |
3 |
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Host cells as platforms for pathogen modulation; Pathogens of the Week #2 |
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Class |
4 |
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Host tissues as platforms for pathogen modulation - IV (Quiz #1: classes 1-3) |
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Class |
5 |
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Basics of pathogen strategies for interactions with host cells and tissues - I; Pathogens of the Week #3 |
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Class |
6 |
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Basics of pathogen strategies for interactions with host cells and tissues - II |
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| Module 2:
| Pathogen Modulation of Host Cell Signaling (Chapters 3, 13-16) |
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Class |
7 |
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Signal Transduction - I; Pathogens of the Week #4 |
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Class |
8 |
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Test # 1 (Classes 1-6) |
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Class |
9 |
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Signal Transduction - II; Pathogens of the Week #5 |
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Class |
10 |
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Toxins – I Introduction, Type III secretion; Type IV secretion |
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Class |
11 |
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Toxins - II; Pathogens of the Week #6 |
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Class |
12 |
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Toxins – III (Quiz #2; classes 1-11) |
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| Module 3:
| Pathogen Manipulation of host cells via adhesion and adhesion-based signaling (Chapters 4-7) |
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Class |
13 |
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Extracellular Matrix and Host Cell Surfaces: Potential Sites of Pathogen Interactions; Pathogens of the Week #7 |
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Class |
14 |
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Bacterial Adherence to Cell Surfaces and Extracellular Matrix |
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Class |
15 |
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Molecular Basis for Cell Adhesion and adhesion-mediated signaling; Pathogens of the Week #8 |
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Class |
16 |
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Test #2 (Classes 1-14) |
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Class |
17 |
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Bacterial signaling to host cells through adhesion molecules and lipid rafts; Pathogens of the Week #9 |
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| Module 4:
| Pathogen manipulation of host cells: establishment of intracellular lifestyles. (Chapters 8-10) |
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Class |
18 |
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Host cell membrane structure and dynamics - I |
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Class |
19 |
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Host cell membrane structure and dynamics - II; Pathogens of the Week #10 |
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Class |
20 |
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Membrane traffic in the endocytic pathway of eukaryotic cells (Quiz #3; classes 1-19) |
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Class |
21 |
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Routes of invasion and selection of an intracellular niche; Pathogens of the Week #11 |
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Class |
22 |
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Selection of an intracellular niche and modulation of intracellular compartments |
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| Module 5:
| Pathogen manipulation of host cell cytoskeleton. (Chapters 11-12) |
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Class |
23 |
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Actin assembly and kinetics; Pathogens of the Week #12 |
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Class |
24 |
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Test #3 (Classes 1-22) |
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Class |
25 |
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Modulation of actin dynamics by actin-binding proteins; Pathogens of the Week #13 |
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Class |
26 |
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Manipulating the cytoskeleton to promote invasion |
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Class |
27 |
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Intracellular motility and intracellular spread; Pathogens of the Week #14 |
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| Module 6:
| Pathogen manipulation of host cells viability. (Chapter 17) |
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Class |
28 |
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Pathogen manipulation of cell death mechanisms |
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Class |
29 |
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Pathogen manipulation of autophagy; Pathogens of the Week #15 |
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Class |
30 |
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Pathogen manipulation of the cell cycle |
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Class |
31 |
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Test #4 (Classes 1-30) |
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