MCB 428
Bacterial Pathogens Lab
School of Molecular & Cellular Biology
University of Illinois at Urbana-Champaign
course home pagecourse contactUniversity of Illinois

 
Fall 2007 Syllabus

Download Hardcopy (.pdf)

Course Instructor:
Gail K. Grabner, Ph.D.
232 Burrill Hall
333-7704
ggrabner@life.uiuc.edu
Office Hours: 10-11 AM, MWF
Teaching Assistant:
Yuka Bannai
bannai@uiuc.edu
Office Hours: Arranged

To request disability accommodations, please speak to the instructor after class or make an appointment by email.

*Asterisk by date indicates that a pre-lab quiz is due that day.

Date Topic Pages
Aug 23 Course orientation, safety, and skills practice 3-13
 
Urinary Tract Infections 14-32
*Aug 28 Day 1: Dipstick test; Gram stain; streak plate
Aug 30 Day 2: Gram stain: oxidase test; biochemical tubes; Sensititre
Sept 4 Day 3: Read results; streak for MBC test
Sept 6 Day 4: Read MBC plates; Discussion questions
 
Quiz 1: September 3 - 8
 
Infections of Gram Positive Cocci 33-58
*Sept 11 Day 1: Throat swab; Strep A test; streak BAP; check blood bottles
Sept 13 Day 2: Normal flora plates; Gram stain; catalase test; coagulase
Sept 18 Day 3: Streptex test, E-tests; phage typing
Sept 20 Day 4: Read phage typing results; Discussion questions
 
Quiz 2: September 17-22
 
Respiratory Tract Infections 59-81
*Sept 25 Day 1: Gram stain; streak plates
Sept 27 Day 2: Normal flora; read results; Gram stain; Enterotube; quad plate
Oct 2 Day 3: Read results; read HAI plates; Discussion questions
 
Quiz 3: September 26 – October 1
     
Oct 4 EXAM 1: Labs 1, 2, and 3  
 
Anaerobic Bacterial Infections 80-102
*Oct 9 Day 1: Gram stain; streak plates
Oct 11 Day 2: Read plate results; Gram stain; quad plate; Toxin A test
Oct 16 Day 3: Read results; Discussion questions
 
Quiz 4: October 10-15
 
Sexually Transmitted Diseases 103-135
*Oct 18 Day 1: Read Gonorrhea, Chlamydia, RPR, HPV, and HSV tests

Oct 23

Day 2: Read HIV ELISA and Western blot
Read HBV tests
Oct 25 Day 3: Record results; Discussion questions
 
Quiz 5: October 22-27
 
Oct 30 EXAM 2: Labs 4 and 5
 
Gastrointestinal Infections 135-158
*Nov 1 Day 1: Streak plates
Formal report topic due
Nov 6 Day 2: Score plates; streak to L-agar; Rota test
Nov 8 Day 3: Gram stain; score plates
Inoculate biochemical tests
Nov 13 Day 4: Score tests; Discussion questions
 
Quiz 6: November 13-16  
 
Nov 15 Exercise on Pathogen Bioinformatics 159
   
Nov 16 Formal Report due by 5:00 pm  
   
Nov 19-23 THANKSGIVING HOLIDAY  
 
Transfer of Antibiotic Resistance Genes 160-194
*Nov 27 Day 1: Mating of donor and recipient
Plasmid profile
Nov 29 Day 2: Check conjugation & pick to growth media
Quantitating plasmid transfer
Conjugative transposons
Dec 4 Day 3: Plasmid prep
Restriction digestion
Dec 6 Day 4: Gel electrophoresis
 
Quiz 7: December 3-7
   
Finals Week EXAM 3: Labs 6, 7, and 8